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clockwork
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CRyPTIC data processing pipelines
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clockwork
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# Clockwork ## Pipelines for processing bacterial sequence data (Illumina only) and variant calling Note: these pipelines were developed for the [CRyPTIC](http://www.crypticproject.org/) project which studies _M. tuberculosis_, but in principle can be used on any bacteria. Clockwork takes fastq input and outputs standard VCF files as output Please see the [clockwork wiki page](https://github.com/iqbal-lab-org/clockwork/wiki) for documentation. ## Citation If you use Clockwork for variant calling please cite the following paper, where it was introduced and benchmarked: "Minos: variant adjudication and joint genotyping of cohorts of bacterial genomes". Hunt et al. Genome Biol. 2022 Jul 5;23(1):147. [doi: 10.1186/s13059-022-02714-x.](https://doi.org/10.1186/s13059-022-02714-x) Whilst Minos is the novel part of the Clockwork variant calling pipeline, it uses several other tools. Please also cite: * Minimap2 https://doi.org/10.1093/bioinformatics/bty191 * SAMtools https://doi.org/10.1093/gigascience/giab008 * Cortex https://doi.org/10.1038/ng.1028 * Trimmomatic: https://doi.org/10.1093/bioinformatics/btu170