cryoet-montage

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SerialEM, Python, and Unity Simulations for Cryo-Electron Tomography and Montage Tomography.

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# Parallel Array Montage Cryo-Tomography/Montage cryo-ET
---

This repository provides resources for the montage tomography with SerialEM.

![Overview of Cryo Montage](https://raw.githubusercontent.com/wright-cemrc-projects/cryoet-montage/main/images/SupplementaryFigure_1_small.png)

## Instructions for Software

1. SerialEM macro documentation to setup a montage tomography data collection.

[Documentation for SerialEM data collections](SerialEM/README.md)

2. TomoGrapher, a tomography simulation program for pre-planning the data collections.

- [TomoGrapher documentation](TomoGrapher/README.md)
	- [TomoGrapher tutorial](TomoGrapher/TomoGrapher_Tutorial.md)

3. Python scripts for stitching images and building stacks for tomograms.

[Python scripts for preprocessing of the tilt series](Python/README.md)

4. Guide for making adjustments to stitching with Etomo and Midas.

[Improving stitching in Etomo with Midas](Midas/README.md)

### Tutorial

Walkthrough in a tutorial with a provided sample dataset collection of a 3x3 montage in the [Tutorial on Montage Creation](Tutorial/README.md)

## References

**Correlative cryogenic montage electron tomography for comprehensive in-situ whole-cell structural studies.** Jie E Yang, Matthew R Larson, Bryan S Sibert, Joseph Y Kim, Daniel Parrell, Juan C Sanchez, Victoria Pappas, Anil Kumar, Kai Cai, Keith J Thompson, Elizabeth R Wright.

bioRxiv 2021.12.31.474669; doi: [https://doi.org/10.1101/2021.12.31.474669](https://doi.org/10.1101/2021.12.31.474669)

cryoet-montage

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