u-inferforce

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u-inferforce (Traction Force Microscopy) is a MATLAB software that reconstructs traction forces of cells adhered on elastic gel doped with beads.

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# u-inferforce
[![DOI](https://zenodo.org/badge/DOI/10.5281/zenodo.11211836.svg)](https://doi.org/10.5281/zenodo.11211836)

![Alt Text](https://raw.githubusercontent.com/DanuserLab/u-inferforce/master/img/TFMThumbnail.jpg)

u-inferforce (previous named Traction Force Microscopy or TFM) is a MATLAB software that reconstructs traction forces of cells adhered on elastic gel doped with beads. The software quantifies the deformation of the gel by image-based tracking of the beads from pairs of images, one displaying in the deformed and displaying the relaxed substrate. Traction force reconstruction is accomplished via L1-regularization, which is particularly good for identifying traction from small adhesions. It generates traction magnitude heat-maps as well as traction vector fields as outputs. The software additionally supports conventional L2-based BEM (Boundary Element Method) and FTTC (Fourier Transform Traction Cytometry).

The innovative parts of the reconstruction algorithms are published in [**Traction microscopy to identify force modulation in subresolution adhesions**](https://doi.org/10.1038/nmeth.3430), *Nature Methods*, 2015, 12, 653–656, written by Sangyoon J Han, Youbean Oak, Alex Groisman, Gaudenz Danuser.

## Software Updates

TFM (Traction Force Microscopy) has been renamed to **u-inferforce**. Please select **u-inferforce** from the analysis packages list to run the software.

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[Software Links](https://github.com/DanuserLab/)

[Han Lab](https://sites.google.com/mtu.edu/hanlab/)

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